Automated Nucleic Acid Sample Quality Control in NGS Workflows


As organic molecules, DNA and RNA are fragile compounds that may endure speedy degradation if not dealt with with warning and saved in optimum situations. Poor high quality of the nucleic acid pattern can have a direct impression on the outcomes of subsequent experiments, together with subsequent technology sequencing (NGS). The preparation of the NGS library is an extended and gradual course of, which usually entails fragmentation of enzymatic or bodily DNA, a number of amplification and ligation reactions, adopted by pattern cleansing steps. As a result of work, specialised chemistry and the evaluation of huge knowledge units, NGS stays an costly course of from begin to end. To keep away from a state of affairs of entry and exit of rubbish and keep away from a waste of money and time, strict high quality management steps are advisable to manage the beginning materials, intermediate merchandise and closing libraries for all preparation package protocols from main supplier libraries. The best high quality management resolution can be straightforward to make use of, economical, versatile and delicate, whereas offering well timed outcomes which can be correct and
of belief Along with the focus and purity of the pattern, the QC outcomes cowl the scale distribution, in addition to the state of degradation and the integrity of the pattern.

Agilent TapeStation programs, primarily based on ScreenTape know-how, provide important benefits that embody distinctive ease of use together with fixed value per pattern. ScreenTape units are prepared to make use of, disposable gels the scale of a bank card that permit computerized separation of as much as 96 samples per evaluation. The power to make use of unused gel traces at a later time ensures a continuing low worth per pattern.

This e-book presents chosen software knowledge and technical notes that describe the advantages of utilizing ScreenTape know-how in NGS workflows.

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